First shot of vaccine may be used in breeders and layers between the age of 2-3 weeks, after priming with live ND vaccines. In broilers it can be injected @ 0.20 ml per bird subcutaneously, during first week of age. In older birds, use 0.30 ml each. The vaccine may be repeated at 8-week interval in breeding stocks, depending on the post-vaccination NDV homologous antibody titres in the flock.