First shot of vaccine may be used in breeders and layers at 2/3-week age. In case of broilers the vaccine can be injected @ 0.20 ml per bird subcutaneously, during first week of age. However, in other birds the dose is 0.30 ml via subcutaneous injection up to 5-week of age and in older birds inject 0.50 ml per bird. The vaccine may be repeated at 8-week interval in breeding stocks.