The vaccine may be used in broilers, breeders, and layers at 8-10 day of age as a primer @ 0.30 ml per bird subcutaneously. In laying birds, subsequent shots of GPVAC ND-Flu can be used at 8-week interval, depending on the antibody levels against ND and AI-H9 in the vaccinated flocks. Because of its inactive form, its usage in the face of ND or H9 outbreak is very safe. Its dose in such circumstances is 0.50 ml per bird.